Additionally, mtDNA induced LTB4 production from macrophages, which may contribute to neutrophil recruitment.Ĭonclusion: We demonstrated here that CBNPs induce acute cell necrosis through lysosomal rupture and that mtDNA released from necrotic cells functions as a key event mediating pulmonary neutrophilic inflammation. Pulmonary neutrophilic inflammation induced by mtDNA was reduced in TLR9 -/- mice. Further analyses suggested that the resulting leakage of mtDNA from the necrotic cells activated neutrophils and triggered severe inflammation in vivo. Necrosis was partly inhibited by a specific lysosomal cathepsin B inhibitor CA074 Me. Macrophages exposed to CBNPs showed necrotic features and were characterized by lysosome rupture, cathepsin B release, reactive oxygen species generation, and reduced intracellular ATP level. Results: In the present study, we found that a single instillation of CBNPs induced neutrophil influx in C57BL/6 mice as early as 4 h post-exposure following the rapid appearance of cell damage indicators in BALF at 30 min. The production of lipid chemoattractant leukotriene B4 (LTB4) in the supernatant and bronchoalveolar lavage fluid (BALF) was quantitated using an enzyme immunoassay (EIA). TLR9-p38 MAPK signaling pathway was detected by Western blotting. Release of mitochondria was observed with Mito Tracker and mitochondrial DNA (mtDNA) was quantified by qPCR via Taqman probes. Cell necrosis and the infiltrated neutrophils in the lungs were detected by flow cytometry. Methods: C57BL/6 mice were intratracheally instilled with carbon black nanoparticles (CBNPs). Previous research indicated a central role of alveolar macrophages in the pathogenesis of particle-related lung disease, but the underlying mechanism remains largely unknown. Carbon nanomaterials are recognized as risk factors for prolonged inflammatory responses and diffuse alveolar injury. Rationale: The adverse health effects of nano-particulate pollutants have attracted much attention in recent years. Select the file that you have just downloaded and select import option Reference Manager (RIS). #Carbon black dispersio in water tween downloadAvailable fromĬlick on Go to download the file. Carbon black nanoparticles induce cell necrosis through lysosomal membrane permeabilization and cause subsequent inflammatory response. Assignors: BELLMANN, GUNTER, BOUSSEL, FRANCOISE, NGUYEN, VAN T.Yuan X, Nie W, He Z, Yang J, Shao B, Ma X, Zhang X, Bi Z, Sun L, Liang X, Tie Y, Liu Y, Mo F, Xie D, Wei Y, Wei X. OF AMERCAN NATIONALITY ASSIGNMENT OF ASSIGNORS INTEREST. OF AMERCAN NATIONALITY reassignment BATTELLE MEMORIAL INSTITUTE, 7, ROUTE DE DRIZE 1227 CAROUGE, GENEVE, SWITZERLAND, A CORP. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority to FR82810295 priority Critical Priority to EP19820810295 priority patent/EP0098338B1/en Application filed by Battelle Memorial Institute Inc filed Critical Battelle Memorial Institute Inc Assigned to BATTELLE MEMORIAL INSTITUTE, 7, ROUTE DE DRIZE 1227 CAROUGE, GENEVE, SWITZERLAND, A CORP. Original Assignee Battelle Memorial Institute Inc Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)īattelle Memorial Institute Geneva Research Center Nguyen Gunter Bellmann Francoise Boussel Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime Application number US06/598,351 Inventor Van T. #Carbon black dispersio in water tween pdfGoogle Patents Low viscosity stable aqueous dispersion of graft carbon blackĭownload PDF Info Publication number US4530961A US4530961A US06/598,351 US59835184A US4530961A US 4530961 A US4530961 A US 4530961A US 59835184 A US59835184 A US 59835184A US 4530961 A US4530961 A US 4530961A Authority US United States Prior art keywords carbon black dispersion water particles grafted Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents US4530961A - Low viscosity stable aqueous dispersion of graft carbon black US4530961A - Low viscosity stable aqueous dispersion of graft carbon black
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